The Rat IgG (Immunoglobulin G) ELISA Kit is meticulously designed to quantitatively analyze the levels of Immunoglobulin G (IgG) in a diverse range of biological samples. Immunoglobulin G is one of the major antibody classes present in the immune system, playing a key role in the defense against pathogens and infections. IgG antibodies are crucial for long-term immune responses, including providing protection against viruses, bacteria, and other invaders. Accurate measurement of IgG levels is vital for understanding immune responses, diagnosing infectious diseases, and monitoring antibody-related conditions. With the Rat IgG ELISA Kit from Assay Genie, researchers can achieve precise and reliable results with exceptional sensitivity and specificity. This kit is manufactured under stringent quality control standards to ensure robust performance, making it an excellent choice for immunological research and immune system studies.
Product Name:
Rat IgG (Immunoglobulin G) ELISA Kit
SKU:
AEES00440
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
