The QuickStep Sheep E3 Estriol ELISA Kit from AssayGenie is a cutting-edge tool for accurately measuring estriol levels in sheep serum, plasma, and other biological samples. This kit boasts high sensitivity and specificity, ensuring precise and reproducible results that are ideal for a variety of research applications.Estriol, a hormone produced by the placenta during pregnancy, plays a vital role in maintaining a healthy pregnancy and fetal development. Monitoring estriol levels is crucial for assessing the health of both the mother and the developing fetus, making this ELISA kit an invaluable tool for reproductive and developmental research.
Whether studying reproductive physiology, investigating pregnancy complications, or developing new diagnostics and treatments, the QuickStep Sheep E3 Estriol ELISA Kit provides researchers with a reliable and accurate way to study estriol levels in sheep samples. Trust AssayGenie for high-quality, innovative solutions for your research needs.
Product Name:
QuickStep Sheep Estriol ELISA Kit
Product Code:
QSES061
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Sheep
Sensitivity:
1.32 pg/mL
Range:
3.13-200 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Estriol
Application:
This ELISA kit applies to the in vitro quantitative determination of E3 concentrations in serum and plasma.
Specificity:
This kit recognizes E3 in samples. No significant cross-reactivity or interference between E3 and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Sheep serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Sheep E3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Sheep E3 are added to the micro ELISA plate wells. Sheep E3 in samples (or standards) competes with a fixed amount of Sheep E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to Sheep E3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Sheep E3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
9.93
20.18
76.59
9.50
19.59
79.59
Standard deviation
0.56
0.94
3.84
0.51
1.13
4.27
CV (%)
5.61
4.66
5.02
5.39
5.76
5.37
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
92-107
98
EDTA plasma(n=8)
92-109
100
Samples were spiked with high concentrations of Sheep E3 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
