The R9AP/RGS9BP Polyclonal Antibody (CAB22557) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to R9AP/RGS9BP, the antibody enables researchers to detect and analyze this important protein in a variety of cell types.R9AP/RGS9BP is essential for the proper functioning of photoreceptor cells in the retina, playing a crucial role in the visual signal transduction cascade. Dysregulation of R9AP/RGS9BP has been implicated in various retinal diseases and vision disorders, making it an attractive target for further research in ophthalmology and vision science.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
R9AP/RGS9BP Polyclonal Antibody
SKU:
CAB22557
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
MARE ECKA LLDG LNKT TACY HHLV LTVG GSAD SQNL RQEL QKTR QKAQ ELAV STCA RLTA VLRD RGLA ADER AEFE RLWV AFSG CLDL LEAD MRRA LELG AAFP LHA
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
R9AP, RGS9, PERRS, R9AP/RGS9BP
Positive Sample:
293T cells transfected with R9AP/RGS9BP
Cellular Localization:
Neuron Projection.
Calculated MW:
25kDa
Observed MW:
30kDa
The protein encoded by this gene functions as a regulator of G protein-coupled receptor signaling in phototransduction. Studies in bovine and mouse show that this gene is expressed only in the retina, and is localized in the rod outer segment membranes. This protein is associated with a heterotetrameric complex, specifically interacting with the regulator of G-protein signaling 9, and appears to function as the membrane anchor for the other largely soluble interacting partners. Mutations in this gene are associated with prolonged electroretinal response suppression (PERRS), also known as bradyopsia.
Purification Method
Affinity purification
Gene ID
388531
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with R9AP/RGS9BP using R9AP/RGS9BP Rabbit pAb (CAB22557) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
