Anti-RPH3AL Antibody (CAB4311)

Antibody Name:	Anti-RPH3AL Antibody
Antibody SKU:	CAB4311
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 161-315 of human RPH3AL (NP_008918.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human
Positive Samples:	BT-474, 22Rv1, SW620, NCI-H460

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 161-315 of human RPH3AL (NP_008918.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KYIL PLKT PGRA DDPH FRPL PTEP AERE PRSS ETSR IYTW ARGR VVSS DSDS DSDL SSSS LEDR LPST GVRD RKGD KPWK ESGG SVEA PRMG FTHP PGHL SGCQ SSLA SGET GTGS ADPP GGPR PGLT RRAP VKDT PGRA PAAD AAPA GPSS CLG
Gene ID:	9501
Uniprot:	Q9UNE2
Cellular Location:	Cytoplasm, Cytoplasmic vesicle, secretory vesicle membrane
Calculated MW:	31kDa/34kDa
Observed MW:	45kDa

Synonyms:	RPH3AL, NOC2
Background:	The protein encoded by this gene plays a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells and plays a key role in insulin secretion by pancreatic cells. This gene is likely a tumor suppressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

UniProt Protein Function:	RPH3AL: Rab GTPase effector involved in the late steps of regulated exocytosis, both in endocrine and exocrine cells. Acts as a potential RAB3B effector protein in epithelial cells. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 17p13.3 Cellular Component: cytoplasm; plasma membrane Molecular Function:calcium ion binding; calcium-dependent phospholipid binding; clathrin binding; cytoskeletal protein binding; protein binding; syntaxin binding Biological Process: calcium ion-dependent exocytosis of neurotransmitter; exocytosis; regulation of calcium ion-dependent exocytosis; vesicle fusion
NCBI Summary:	The protein encoded by this gene plays a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells and plays a key role in insulin secretion by pancreatic cells. This gene is likely a tumor suppressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jun 2010]
UniProt Code:	Q9UNE2
NCBI GenInfo Identifier:	74735140
NCBI Gene ID:	9501
NCBI Accession:	Q9UNE2.1
UniProt Secondary Accession:	Q9UNE2,Q9BSB3, D3DTG7,
UniProt Related Accession:	Q9UNE2
Molecular Weight:	31,190 Da
NCBI Full Name:	Rab effector Noc2
NCBI Synonym Full Names:	rabphilin 3A-like (without C2 domains)
NCBI Official Symbol:	RPH3AL
NCBI Official Synonym Symbols:	NOC2
NCBI Protein Information:	rab effector Noc2
UniProt Protein Name:	Rab effector Noc2
UniProt Synonym Protein Names:	No C2 domains protein; Rabphilin-3A-like protein
Protein Family:	Rab effector
UniProt Gene Name:	RPH3AL
UniProt Entry Name:	RPH3L_HUMAN

	Western blot analysis of extracts of various cell lines, using RPH3AL antibody (CAB4311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver using RPH3AL antibody (CAB4311) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using RPH3AL antibody (CAB4311) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)