Anti-RAB21 Antibody (CAB12095)

Antibody Name:	Anti-RAB21 Antibody
Antibody SKU:	CAB12095
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human RAB21 (NP_055814.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, 293T, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human RAB21 (NP_055814.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAA GGGG GGAA AAGR AYSF KVVL LGEG CVGK TSLV LRYC ENKF NDKH ITTL QASF LTKK LNIG GKRV NLAI WDTA GQER FHAL GPIY YRDS NGAI LVYD ITDE DSFQ KVKN WVKE LRKM LGNE ICLC IVGN KIDL EKER HVSI QEAE SYAE SVGA KHYH TSAK QNKG IEEL FLDL CKRM IETA QVDE RAKG NGSS QPGT ARRG VQII DDEP QAQT SGGG CCSS G
Gene ID:	23011
Uniprot:	Q9UL25
Cellular Location:	Cleavage furrow, Cytoplasmic vesicle membrane, Early endosome membrane, Endoplasmic reticulum membrane, Golgi apparatus, Golgi apparatus membrane, Lipid-anchor, trans-Golgi network
Calculated MW:	24kDa
Observed MW:	24kDa

Synonyms:

RAB21

Background:

This gene belongs to the Rab family of monomeric GTPases, which are involved in the control of cellular membrane traffic. The encoded protein plays a role in the targeted trafficking of integrins via its association with integrin alpha tails. As a consequence, the encoded protein is involved in the regulation of cell adhesion and migration. Expression of this gene is associated with a poor prognosis for glioma patients. This gene is downregulated by the tumor suppressor miR-200b, and miRNA-200b is itself downregulated in glioma tissues.

UniProt Protein Function:	RAB21: Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration. During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis. Belongs to the small GTPase superfamily. Rab family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, Rab Chromosomal Location of Human Ortholog: 12q21.1 Cellular Component: endosome; focal adhesion; internal side of plasma membrane; trans-Golgi network; vesicle membrane Molecular Function:GDP binding; GTP binding; GTPase activity; protein binding Biological Process: positive regulation of receptor-mediated endocytosis; regulation of exocytosis
NCBI Summary:	This gene belongs to the Rab family of monomeric GTPases, which are involved in the control of cellular membrane traffic. The encoded protein plays a role in the targeted trafficking of integrins via its association with integrin alpha tails. As a consequence, the encoded protein is involved in the regulation of cell adhesion and migration. Expression of this gene is associated with a poor prognosis for glioma patients. This gene is downregulated by the tumor suppressor miR-200b, and miRNA-200b is itself downregulated in glioma tissues. [provided by RefSeq, Nov 2015]
UniProt Code:	Q9UL25
NCBI GenInfo Identifier:	13633613
NCBI Gene ID:	23011
NCBI Accession:	Q9UL25.3
UniProt Secondary Accession:	Q9UL25,Q14466, Q569H3,
UniProt Related Accession:	Q9UL25
Molecular Weight:	24,348 Da
NCBI Full Name:	Ras-related protein Rab-21
NCBI Synonym Full Names:	RAB21, member RAS oncogene family
NCBI Official Symbol:	RAB21
NCBI Protein Information:	ras-related protein Rab-21
UniProt Protein Name:	Ras-related protein Rab-21
Protein Family:	Ras-related protein
UniProt Gene Name:	RAB21
UniProt Entry Name:	RAB21_HUMAN

	Western blot analysis of extracts of various cell lines, using RAB21 antibody (CAB12095) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of C6 cells using RAB21 antibody (CAB12095) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using RAB21 antibody (CAB12095) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using RAB21 antibody (CAB12095) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using RAB21 antibody (CAB12095) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)