Rab5 Antibody is a rabbit polyclonal antibody directed against Rab5, offered by Assay Genie for research applications. Reported applications include WB, IHC and ICC/IF. Reported reactivity: Human, Mouse, Rat, Bovine and Monkey. Purified by Protein A.
Rab5 is a 24 kDa GTPase that regulates early endosome dynamics and endocytic trafficking. It is ubiquitously expressed and localizes to early endosomes and the plasma membrane. Rab5 controls the fusion of endocytic vesicles with early endosomes and mediates homotypic endosome fusion. It recruits effectors such as EEA1 and Rabaptin-5 to facilitate vesicle docking and fusion. In the nervous system, Rab5 is essential for receptor internalization, synaptic vesicle recycling, and neurotrophin signaling. Dysregulation of Rab5 activity can lead to endosomal dysfunction, impaired signaling, and neurodegeneration. It is widely used as a marker of early endosomes and is a key player in maintaining neuronal homeostasis. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
Product Name:
Rab5 Antibody
Product SKU:
STPA00025
Size:
100 µg
Target:
Rab5
Host Species:
Rabbit
Antibody Type:
Polyclonal
Research Areas:
Cell Signaling, Tags and Cell Markers, Organelle Markers, Protein Trafficking, Vesicle Transport, Neuroscience, Cell Markers, Neuron Markers, Presynaptic Markers
Buffer:
PBS, 50% glycerol, 0.09% sodium azide
Storage & Shipping:
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:
Liquid
Applications:
WB, IHC, ICC/IF
Purification:
Protein A Purified
Concentration:
1 mg/ml
Reactivity:
Human, Mouse, Rat, Bovine, Monkey
Recommended Dilution:
WB (1:1000), IHC (1:100), ICC/IF (1:80); optimal dilutions for assays should be determined by the user.
Specificity:
Detects ~26kDa.
Guarantee:
12 months from date of dispatch
Immunogen:
Human Rab5 synthetic peptide conjugated to KLH; identical to dog Rab5 sequence over the residues
Immunogen Species:
Human
Accession Number:
NP_004153.2
Gene ID:
5868
Swiss-Prot:
P20339
Cellular Localization:
Cell Membrane | Endosome | Early Endosome Membrane | Melanosome
Alternative Names:
Rab 5A, RAS associated protein RAB5A, Ras related protein Rab 5 A
References:
1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Sonnichsen B., et al. (2000) J. Cell Biol. 149: 901-913 6. Woodman P.G. (2000) Traffic. 1: 695-701.
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