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Anti-RAB5B Antibody (CAB7447)

SKU:
CAB7447
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 11 Anti-RAB5B Antibody CAB7447
  • Cell Biology Antibodies 11 Anti-RAB5B Antibody CAB7447
  • Cell Biology Antibodies 11 Anti-RAB5B Antibody CAB7447
€139 - €419
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Description

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Antibody Name:Anti-RAB5B Antibody
Antibody SKU:CAB7447
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RAB5B (NP_002859.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, SKOV3, Mouse ovary, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RAB5B (NP_002859.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTSR STAR PNGQ PQAS KICQ FKLV LLGE SAVG KSSL VLRF VKGQ FHEY QEST IGAA FLTQ SVCL DDTT VKFE IWDT AGQE RYHS LAPM YYRG AQAA IVVY DITN QETF ARAK TWVK ELQR QASP SIVI ALAG NKAD LANK RMVE YEEA QAYA DDNS LLFM ETSA KTAM NVND LFLA IAKK LPKS EPQN LGGA AGRS RGVD LHEQ SQQN KSQC CSN
Gene ID:5869
Uniprot:P61020
Cellular Location:Cell membrane, Cytoplasmic side, Early endosome membrane, Lipid-anchor, Melanosome
Calculated MW:19kDa/23kDa
Observed MW:24kDa
Synonyms:RAB5B
Background:
UniProt Protein Function:RAB5B: Protein transport. Probably involved in vesicular traffic. Belongs to the small GTPase superfamily. Rab family.
UniProt Protein Details:

Protein type:G protein; G protein, monomeric, Rab; G protein, monomeric

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: membrane; endocytic vesicle; early endosome membrane; early endosome; plasma membrane; melanosome; intracellular; endosome

Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; GTP-dependent protein binding

Biological Process: antigen processing and presentation; intracellular protein transport; endosome organization and biogenesis; metabolic process; regulation of endocytosis; plasma membrane to endosome transport; Rab protein signal transduction

UniProt Code:P61020
NCBI GenInfo Identifier:46577637
NCBI Gene ID:5869
NCBI Accession:P61020.1
UniProt Related Accession:P61020
Molecular Weight:
NCBI Full Name:Ras-related protein Rab-5B
NCBI Synonym Full Names:RAB5B, member RAS oncogene family
NCBI Official Symbol:RAB5B  
NCBI Protein Information:ras-related protein Rab-5B
UniProt Protein Name:Ras-related protein Rab-5B
Protein Family:Ras-related protein
UniProt Gene Name:RAB5B  
UniProt Entry Name:RAB5B_HUMAN
Anti-RAB5B Antibody (CAB7447)Western blot analysis of extracts of various cell lines, using RAB5B antibody (CAB7447) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
Anti-RAB5B Antibody (CAB7447)Immunofluorescence analysis of L929 cells using RAB5B antibody (CAB7447) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-RAB5B Antibody (CAB7447)Immunofluorescence analysis of human colon carcinoma using RAB5B antibody (CAB7447) at dilution of 1:100. Blue: DAPI for nuclear staining.

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