The RAB5B Antibody (CAB7447) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically recognizes Rab5B in human samples and has been validated for use in Western blot applications.Rab5B plays a crucial role in controlling vesicle fusion and trafficking within cells, making it essential for various cellular processes such as receptor internalization, protein sorting, and organelle dynamics. Dysregulation of Rab5B has been linked to diseases like cancer, neurodegenerative disorders, and infectious diseases, highlighting its importance in cellular homeostasis.
This antibody is validated for use in WB, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RAB5B Antibody
SKU:
CAB7447
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
YDIT NQET FARA KTWV KELQ RQAS PSIV IALA GNKA DLAN KRMV EYEE AQAY ADDN SLLF METS AKTA MNVN DLFL AIAK KLPK SEPQ NLGG AAGR SRGV D
Tested Applications:
WBIF/ICCELISAIF-P
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:200
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RAB5B
Positive Sample:
HeLa, SKOV3, Mouse ovary, Rat brain
Cellular Localization:
Cell Membrane, Cytoplasmic Side, Early Endosome Membrane, Lipid-Anchor, Melanosome.
Calculated MW:
24kDa
Observed MW:
24kDa
Enables GDP binding activity; GTP-dependent protein binding activity; and GTPase activity. Involved in antigen processing and presentation and plasma membrane to endosome transport. Located in endosome and extracellular exosome.
Purification Method
Affinity purification
Gene ID
5869
RRID
AB_2767978
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using RAB5B Rabbit pAb (CAB7447) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
Immunofluorescence analysis of L929 cells using RAB5B Rabbit pAb (CAB7447) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
