The Rabbit anti HA-Tag pAb (CABE036) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and sensitive, making it ideal for a variety of applications including immunoprecipitation, immunofluorescence, and flow cytometry.The HA tag is commonly used to facilitate the identification and purification of recombinant proteins in cell biology and molecular biology research.
This antibody is validated for use in WB, IF/ICC applications and has demonstrated reactivity against Species independent samples.
Product Name:
Rabbit anti HA-Tag pAb
SKU:
CABE036
Size:
50μL, 100μL, 200μL
Reactivity:
Species independent
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
YPVD VPDY A
Tested Applications:
WBIF/ICC
Recommended Dilution:
WB
1:2000 - 1:6000
IF
1:50 - 1:200
Synonyms:
HA, HA tag, HA-tag
Positive Sample:
293T
Observed MW:
26kDa
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method
Affinity purification
RRID
AB_2771924
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells, using Rabbit anti HA-Tag pAb (CABE036) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunofluorescence analysis of 293T-HA-C and 293T-HA-N cells using Rabbit anti HA-Tag pAb (CABE036) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
