Rabbit Insulin Like Growth Factor 2 (IGF2) ELISA Kit
The Rabbit Insulin-like Growth Factor 2 (IGF2) ELISA Kit is a powerful tool for the accurate measurement of IGF2 levels in rabbit serum, plasma, and tissue culture samples. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.IGF2 is a key regulatory protein that plays a critical role in growth and development, as well as in various disease processes such as cancer.
By accurately measuring IGF2 levels, researchers can gain valuable insights into the underlying mechanisms of these conditions and develop novel therapeutic strategies.Overall, the Rabbit IGF2 ELISA Kit is an indispensable resource for researchers studying the role of IGF2 in health and disease, offering reliable and reproducible data for advancing scientific knowledge and driving innovative research initiatives.
Product Name:
Rabbit Insulin Like Growth Factor 2 (IGF2) ELISA Kit
Product Code:
RBDL00030
Size:
96 Assays
Target:
IGF2
Synonyms:
INSIGF, IGF-II, Somatomedin A, Preptin, Insulin-like growth factor II Ala-25 Del
Detection Method:
Sandwich
Range:
4.687-300ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
