The Rabbit 5-HT (5-Hydroxytryptamine) ELISA Kit is specifically designed for the quantitative detection of 5-HT levels in rabbit serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and accuracy, providing researchers with dependable and consistent results for their studies.5-Hydroxytryptamine, also known as serotonin, is a neurotransmitter that plays a critical role in regulating mood, appetite, and sleep. Imbalances in serotonin levels have been linked to various mental health disorders such as depression, anxiety, and schizophrenia.
This ELISA kit serves as a valuable tool for investigating the role of serotonin in these conditions and potentially identifying new therapeutic targets. With its user-friendly protocol and superior performance, the Rabbit 5-HT ELISA Kit is an essential resource for researchers working in neuroscience, psychiatry, and drug development. Unlock the potential of your research with this high-quality kit from Assay Genie.
Product Name:
Rabbit ST/5-HT (5-hydroxytryptamine) ELISA Kit
SKU:
AEFI00843
Reactivity:
Rabbit
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
0.938 ng/mL
Range:
1.563-100 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 10)
89-100%
85-102%
88-105%
EDTA Plasma (n = 10)
85-101%
82-94%
93-100%
Heparin Plasma (n = 10)
80-93%
82-95%
84-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 10)
93-100
95
EDTA Plasma (n = 10)
86-101
93
Heparin Plasma (n = 10)
81-98
96
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
