Anti-RAC2 Antibody (CAB1139)

Antibody Name:	Anti-RAC2 Antibody
Antibody SKU:	CAB1139
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human RAC2 (NP_002863.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse spleen, Mouse lung, Rat lung, Rat spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human RAC2 (NP_002863.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MQAI KCVV VGDG AVGK TCLL ISYT TNAF PGEY IPTV FDNY SANV MVDS KPVN LGLW DTAG QEDY DRLR PLSY PQTD VFLI CFSL VSPA SYEN VRAK WFPE VRHH CPST PIIL VGTK LDLR DDKD TIEK LKEK KLAP ITYP QGLA LAKE IDSV KYLE CSAL TQRG LKTV FDEA IRAV LCPQ PTRQ QKRA CSLL
Gene ID:	5880
Uniprot:	P15153
Cellular Location:	Cytoplasm
Calculated MW:	21kDa
Observed MW:	21KDa

Synonyms:

RAC2, EN-7, Gx, HSPC022, p21-Rac2

Background:

This gene encodes a member of the Ras superfamily of small guanosine triphosphate (GTP)-metabolizing proteins. The encoded protein localizes to the plasma membrane, where it regulates diverse processes, such as secretion, phagocytosis, and cell polarization. Activity of this protein is also involved in the generation of reactive oxygen species. Mutations in this gene are associated with neutrophil immunodeficiency syndrome. There is a pseudogene for this gene on chromosome 6.

UniProt Protein Function:	RAC2: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. Interacts with DOCK2, which may activate it. Hematopoietic specific. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. Belongs to the small GTPase superfamily. Rho family.
UniProt Protein Details:	Protein type:G protein, monomeric, Rho; Motility/polarity/chemotaxis; G protein, monomeric; G protein Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: actin filament; cytosol; focal adhesion; lamellipodium; phagocytic vesicle membrane; plasma membrane Molecular Function:GTP binding Biological Process: actin filament organization; platelet activation; regulation of hydrogen peroxide metabolic process; regulation of small GTPase mediated signal transduction; signal transduction Disease: Neutrophil Immunodeficiency Syndrome
NCBI Summary:	This gene encodes a member of the Ras superfamily of small guanosine triphosphate (GTP)-metabolizing proteins. The encoded protein localizes to the plasma membrane, where it regulates diverse processes, such as secretion, phagocytosis, and cell polarization. Activity of this protein is also involved in the generation of reactive oxygen species. Mutations in this gene are associated with neutrophil immunodeficiency syndrome. There is a pseudogene for this gene on chromosome 6. [provided by RefSeq, Jul 2013]
UniProt Code:	P15153
NCBI GenInfo Identifier:	131806
NCBI Gene ID:	5880
NCBI Accession:	P15153.1
UniProt Secondary Accession:	P15153,Q9UDJ4,
UniProt Related Accession:	P15153,AAB22207
Molecular Weight:	21,429 Da
NCBI Full Name:	Ras-related C3 botulinum toxin substrate 2
NCBI Synonym Full Names:	ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
NCBI Official Symbol:	RAC2
NCBI Official Synonym Symbols:	Gx; EN-7; HSPC022; p21-Rac2
NCBI Protein Information:	ras-related C3 botulinum toxin substrate 2
UniProt Protein Name:	Ras-related C3 botulinum toxin substrate 2
UniProt Synonym Protein Names:	GX; Small G protein; p21-Rac2
Protein Family:	Ras-related protein
UniProt Gene Name:	RAC2
UniProt Entry Name:	RAC2_HUMAN

	Western blot analysis of extracts of Mouse spleen, using RAC2 Polyclonal Antibody (CAB1139) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Western blot analysis of extracts of various cell lines, using RAC2 Polyclonal Antibody (CAB1139) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Immunohistochemistry of paraffin-embedded rat brain using RAC2 antibody (CAB1139) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using RAC2 antibody (CAB1139) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using RAC2 antibody (CAB1139) at dilution of 1:100 (40x lens).

Antibodies

RAC2 Antibody (PACO11686)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)