The Rad23A Monoclonal Antibody (CAB5147) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using rabbit monoclonal technology, offers high specificity and sensitivity for detecting Rad23A in human samples, making it an excellent choice for Western blot and immunohistochemistry applications.Rad23A is a multifunctional protein that plays a crucial role in maintaining genomic stability and cellular homeostasis by facilitating DNA damage recognition and removal of misfolded proteins. Dysregulation of Rad23A has been linked to various human diseases, including cancer and neurodegenerative disorders.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Rad23A Monoclonal Antibody
SKU:
CAB5147
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1271
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HR23A, HHR23A, Rad23A
Positive Sample:
HeLa, Jurkat, U-87MG
Cellular Localization:
Nucleus.
Calculated MW:
40kDa
Observed MW:
50kDa
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair. Proteins in this family have a modular domain structure consisting of an ubiquitin-like domain (UbL), ubiquitin-associated domain 1 (UbA1), XPC-binding domain and UbA2. The protein encoded by this gene plays an important role in nucleotide excision repair and also in delivery of polyubiquitinated proteins to the proteasome. Alternative splicing results in multiple transcript variants encoding multiple isoforms.
Purification Method
Affinity purification
Gene ID
5886
RRID
AB_2863466
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Rad23A Rabbit mAb (CAB5147) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1min.
Immunofluorescence analysis of NIH-3T3 cells using Rad23A Rabbit mAb (CAB5147) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
