Anti-Rad23B Antibody (CAB1034)

Antibody Name:	Anti-Rad23B Antibody
Antibody SKU:	CAB1034
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Rad23B (NP_002865.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, Jurkat, SKOV3, Mouse testis, Mouse brain, Mouse liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Rad23B (NP_002865.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GKIL NDDT ALKE YKID EKNF VVVM VTKP KAVS TPAP ATTQ QSAP ASTT AVTS STTT TVAQ APTP VPAL APTS TPAS ITPA SATA SSEP APAS AAKQ EKPA E
Gene ID:	5887
Uniprot:	P54727
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	35kDa/43kDa
Observed MW:	80kDa

Synonyms:

RAD23B, HHR23B, HR23B, P58

Background:

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

UniProt Protein Function:	RAD23B: Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum- associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. Belongs to the RAD23 family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 9q31.2 Cellular Component: proteasome complex; nucleoplasm; cytoplasm; nucleus Molecular Function:protein binding; damaged DNA binding; polyubiquitin binding; single-stranded DNA binding Biological Process: nucleotide-excision repair, DNA damage recognition; proteasomal ubiquitin-dependent protein catabolic process; nucleotide-excision repair; regulation of proteasomal ubiquitin-dependent protein catabolic process; spermatogenesis; nucleotide-excision repair, DNA damage removal; DNA repair
NCBI Summary:	The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011]
UniProt Code:	P54727
NCBI GenInfo Identifier:	1709985
NCBI Gene ID:	5887
NCBI Accession:	P54727.1
UniProt Secondary Accession:	P54727,Q7Z5K8, Q8WUB0, B3KWK8, G5E9P0,
UniProt Related Accession:	P54727
Molecular Weight:	409
NCBI Full Name:	UV excision repair protein RAD23 homolog B
NCBI Synonym Full Names:	RAD23 homolog B (S. cerevisiae)
NCBI Official Symbol:	RAD23B
NCBI Official Synonym Symbols:	P58; HR23B; HHR23B
NCBI Protein Information:	UV excision repair protein RAD23 homolog B; RAD23, yeast homolog of, B; XP-C repair complementing protein; XP-C repair complementing complex 58 kDa; XP-C repair-complementing complex 58 kDa protein
UniProt Protein Name:	UV excision repair protein RAD23 homolog B
UniProt Synonym Protein Names:	XP-C repair-complementing complex 58 kDa protein; p58
Protein Family:	Ubiquitin receptor
UniProt Gene Name:	RAD23B
UniProt Entry Name:	RD23B_HUMAN

	Western blot analysis of extracts of various cell lines, using Rad23B antibody (CAB1034) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon using Rad23B Rabbit pAb (CAB1034) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using Rad23B Rabbit pAb (CAB1034) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using Rad23B antibody (CAB1034) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-Rad23B Antibody (CAB20000)[KO Validated]

RAD23B Antibody (PACO06789)

RAD23B Antibody (PACO11693)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)