Western blot analysis of extracts of various cell lines, using Rad51D antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
The Rad51D Monoclonal Antibody (CAB20869) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in mice, is highly specific to human RAD51D and has been validated for use in multiple applications, including Western blot and immunofluorescence.RAD51D is essential for homologous recombination, a critical mechanism for DNA repair and maintenance of genomic stability. Dysregulation of RAD51D has been linked to various diseases, including cancer and genetic disorders. By targeting RAD51D, researchers can gain insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Rad51D Monoclonal Antibody
SKU:
CAB20869
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2800
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TRAD, R51H3, BROVCA4, RAD51L3, Rad51D
Positive Sample:
293T, Hep G2, Mouse testis, Rat thymus
Cellular Localization:
Chromosome, Cytoplasm, Nucleus, Centrosome, Cytoskeleton, Microtubule Organizing Center, Telomere.
Calculated MW:
35kDa
Observed MW:
35kDa
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinational repair of DNA. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream ring finger and FYVE-like domain containing 1 (RFFL) gene.
Purification Method
Affinity purification
Gene ID
5892
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Rad51D Rabbit mAb (CAB20869) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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