Anti-RAP1A Antibody (CAB0975)

Antibody Name:	Anti-RAP1A Antibody
Antibody SKU:	CAB0975
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human RAP1A (NP_002875.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HL-60, Mouse lung, Mouse kidney, Rat kidney, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human RAP1A (NP_002875.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QCML EILD TAGT EQFT AMRD LYMK NGQG FALV YSIT AQST FNDL QDLR EQIL RVKD TEDV PMIL VGNK CDLE DERV VGKE QGQN LARQ WCNC AFLE SSAK S
Gene ID:	5906
Uniprot:	P62834
Cellular Location:	Cell junction, Cell membrane, Cytoplasm, Early endosome, Lipid-anchor, perinuclear region
Calculated MW:	20kDa
Observed MW:	21kDa

Synonyms:

RAP1A, C21KG, G-22K, KREV-1, KREV1, RAP1, SMGP21

Background:

This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	RAP1A: a small G protein of the Ras family with tumor suppressor activity. Induces morphological reversion of a cell line transformed by a Ras oncogene. Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Interacts with SGSM1, SGSM2 and SGSM3.
UniProt Protein Details:	Protein type:Tumor suppressor; G protein, monomeric; Motility/polarity/chemotaxis; G protein; G protein, monomeric, Ras Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: neuron projection; perinuclear region of cytoplasm; late endosome; cytoplasm; early endosome; plasma membrane; cell junction; cytosol Molecular Function:GTPase activity; Rap guanyl-nucleotide exchange factor activity; protein binding; Ras GTPase binding; GTP binding; protein complex binding; protein transporter activity Biological Process: platelet activation; microvillus biogenesis; protein transport; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of protein kinase activity; energy reserve metabolic process; blood coagulation; signal transduction; regulation of insulin secretion; Rap protein signal transduction
NCBI Summary:	This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Code:	P62834
NCBI GenInfo Identifier:	51338607
NCBI Gene ID:	5906
NCBI Accession:	P62834.1
UniProt Secondary Accession:	P62834,P10113,
UniProt Related Accession:	P62834
Molecular Weight:	184
NCBI Full Name:	Ras-related protein Rap-1A
NCBI Synonym Full Names:	RAP1A, member of RAS oncogene family
NCBI Official Symbol:	RAP1A
NCBI Official Synonym Symbols:	RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21
NCBI Protein Information:	ras-related protein Rap-1A; Ras-related protein Krev-1; GTP-binding protein smg p21A
UniProt Protein Name:	Ras-related protein Rap-1A
UniProt Synonym Protein Names:	C21KG; G-22K; GTP-binding protein smg p21A; Ras-related protein Krev-1
Protein Family:	Ras-related protein
UniProt Gene Name:	RAP1A
UniProt Entry Name:	RAP1A_HUMAN

	Western blot analysis of extracts of various cell lines, using RAP1A antibody (CAB0975) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human liver cancer using RAP1A/RAP1B antibody (CAB0975) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using RAP1A/RAP1B antibody (CAB0975) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using RAP1A/RAP1B antibody (CAB0975) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
RAP1A Antibody (PACO11715)	Human RAP1A ELISA Kit
	Human Ras-related protein Rap-1A (RAP1A) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)