Anti-RASSF5 Antibody (CAB7389)

Antibody Name:	Anti-RASSF5 Antibody
Antibody SKU:	CAB7389
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-265 of human RASSF5 (NP_872606.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1, A-549, 293T, Jurkat, Mouse brain, Mouse liver, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-265 of human RASSF5 (NP_872606.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTVD SSMS SGYC SLDE ELED CFFT AKTT FFRN AQSK HLSK NVCK PVEE TQRP PTLQ EIKQ KIDS YNTR EKNC LGMK LSED GTYT GFIK VHLK LRRP VTVP AGIR PQSI YDAI KEVN LAAT TDKR TSFY LPLD AIKQ LHIS STTT VSEV IQGL LKKF MVVD NPQK FALF KRIH KDGQ VLFQ KLSI ADRP LYLR LLAG PDTE VLSF VLKE NETG EVEW DAFS IPEL QNFL TILE KEEQ DKIQ QVQK KYDK FRQK LEEA LRES QGKP G
Gene ID:	83593
Uniprot:	Q8WWW0
Cellular Location:	Cytoplasm, cytoskeleton
Calculated MW:	30kDa/37kDa/47kDa
Observed MW:	37kDa, 45kDa

Synonyms:

RASSF5, Maxp1, NORE1, NORE1A, NORE1B, RAPL, RASSF3

Background:

This gene is a member of the Ras association domain family. It functions as a tumor suppressor, and is inactivated in a variety of cancers. The encoded protein localizes to centrosomes and microtubules, and associates with the GTP-activated forms of Ras, Rap1, and several other Ras-like small GTPases. The protein regulates lymphocyte adhesion and suppresses cell growth in response to activated Rap1 or Ras. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	RASSF5: Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing. May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS1 and KRAS induced apoptosis. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Autophagy; Cytoskeletal; Motility/polarity/chemotaxis; Tumor suppressor Chromosomal Location of Human Ortholog: 1q32.1 Molecular Function:protein binding
NCBI Summary:	This gene is a member of the Ras association domain family. It functions as a tumor suppressor, and is inactivated in a variety of cancers. The encoded protein localizes to centrosomes and microtubules, and associates with the GTP-activated forms of Ras, Rap1, and several other Ras-like small GTPases. The protein regulates lymphocyte adhesion and suppresses cell growth in response to activated Rap1 or Ras. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8WWW0
NCBI GenInfo Identifier:	74751587
NCBI Gene ID:	83593
NCBI Accession:	Q8WWW0.1
UniProt Secondary Accession:	Q8WWW0,Q5SY32, Q8WWV9, Q8WXF4, Q9BT99, A8K1E6,
UniProt Related Accession:	Q8WWW0
Molecular Weight:	47kDa
NCBI Full Name:	Ras association domain-containing protein 5
NCBI Synonym Full Names:	Ras association domain family member 5
NCBI Official Symbol:	RASSF5
NCBI Official Synonym Symbols:	RAPL; Maxp1; NORE1; NORE1A; NORE1B; RASSF3
NCBI Protein Information:	ras association domain-containing protein 5
UniProt Protein Name:	Ras association domain-containing protein 5
UniProt Synonym Protein Names:	New ras effector 1; Regulator for cell adhesion and polarization enriched in lymphoid tissues; RAPL
Protein Family:	Ras association domain-containing protein
UniProt Gene Name:	RASSF5

	Western blot analysis of extracts of various cell lines, using RASSF5 antibody (CAB7389) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human prostate using RASSF5 antibody (CAB7389) at dilution of 1:100 (40x lens).

Antibodies

Anti-RASSF5 Antibody (CAB12602)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)