Rat A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) ELISA Kit
The Rat ADAMTS7 (A Disintegrin and Metalloproteinase with Thrombospondin-7) ELISA Kit is a powerful tool for the accurate detection of ADAMTS7 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications.ADAMTS7 is a key enzyme involved in extracellular matrix remodeling and plays a crucial role in various physiological and pathological processes, including arthritis, atherosclerosis, and cancer.
By measuring ADAMTS7 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.With its advanced technology and user-friendly design, the Rat ADAMTS7 ELISA Kit is an indispensable resource for researchers studying ADAMTS7 biology and its implications in disease. Get accurate and reliable results with this cutting-edge kit from Assay Genie.
Product Name:
Rat A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) ELISA Kit
Product Code:
RTDL00024
Size:
96 Assays
Target:
ADAMTS7
Synonyms:
COMPase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
