The Rat AChE (Acetylcholinesterase) CLIA Kit is a reliable and accurate assay kit designed for the quantitative measurement of acetylcholinesterase levels in rat samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Acetylcholinesterase is an important enzyme involved in the regulation of neurotransmission, particularly in the cholinergic system. Dysregulation of acetylcholinesterase activity has been linked to various neurological disorders such as Alzheimer's disease, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its user-friendly protocol and rapid assay time, the Rat AChE CLIA Kit is an essential tool for researchers studying the role of acetylcholinesterase in neurological function and exploring potential therapeutic strategies for related disorders.
Product Name:
Rat AChE (Acetylcholinesterase) CLIA Kit
SKU:
RTES00193
Target:
Rat AChE (Acetylcholinesterase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AChE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AChE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AChE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat AChE. You can calculate the concentration of Rat AChE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
85-100
92-105
Average (%)
101
92
98
1:4
Range (%)
84-97
88-102
93-109
Average (%)
91
94
100
1:8
Range (%)
89-101
88-102
89-101
Average (%)
96
95
95
1:16
Range (%)
100-117
90-102
86-97
Average (%)
108
97
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
94-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.25
4.99
0.53
1.27
4.71
Standard deviation
0.05
0.09
0.51
0.07
0.09
0.49
C V (%)
9.26
7.2
10.22
13.21
7.09
10.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat AChE concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat AChE in samples. No significant cross-reactivity or interference between Rat AChE and analogues was observed.
