The Human AChE (Acetylcholinesterase) CLIA Kit is specially designed for the accurate measurement of AChE levels in human samples like serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.AChE is an essential enzyme that plays a critical role in the nervous system by breaking down the neurotransmitter acetylcholine. Dysregulation of AChE activity has been implicated in various neurological disorders such as Alzheimer's disease and Parkinson's disease, highlighting its importance as a potential biomarker for understanding and treating these conditions.
With its reliable performance and versatile applications, the Human AChE CLIA Kit is an invaluable tool for researchers studying neurodegenerative diseases and exploring potential therapeutic interventions.
Product Name:
Human AChE (Acetylcholinesterase) CLIA Kit
SKU:
HUES00187
Target:
Human AChE (Acetylcholinesterase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AChE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AChE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AChE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AChE. You can calculate the concentration of Human AChE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-119
86-96
95-108
Average (%)
110
91
101
1:4
Range (%)
96-108
93-105
93-108
Average (%)
101
98
100
1:8
Range (%)
99-115
100-115
102-118
Average (%)
108
108
108
1:16
Range (%)
89-99
98-114
99-111
Average (%)
94
105
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
91
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
2.33
9.79
1.15
2.27
9.26
Standard deviation
0.12
0.25
0.93
0.11
0.16
1.06
C V (%)
11.01
10.73
9.5
9.57
7.05
11.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AChE concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AChE in samples. No significant cross-reactivity or interference between Human AChE and analogues was observed.
