The Rat AGRP (Agouti-Related Protein) CLIA Kit is a highly sensitive assay kit designed for the accurate detection of AGRP levels in rat serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for a variety of research applications.AGRP is a key protein involved in regulating food intake and energy balance, making it a valuable biomarker for studying obesity, metabolic disorders, and other related conditions.
By accurately measuring AGRP levels, researchers can gain valuable insights into the mechanisms underlying these conditions and develop potential therapeutic strategies.Overall, the Rat AGRP CLIA Kit is a powerful tool for researchers looking to investigate the role of AGRP in various physiological processes and diseases, offering high sensitivity and specificity for precise and dependable results.
Product Name:
Rat AgRP (Agouti Related Protein) CLIA Kit
SKU:
RTES00630
Target:
Rat AgRP (Agouti Related Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AgRP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AgRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AgRP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat AgRP. You can calculate the concentration of Rat AgRP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-114
94-106
97-110
Average (%)
108
100
103
1:4
Range (%)
89-104
99-115
90-105
Average (%)
96
108
96
1:8
Range (%)
96-110
96-108
95-106
Average (%)
102
102
101
1:16
Range (%)
89-104
99-115
93-106
Average (%)
96
106
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
91-107
98
Cell culture media (n=5)
100-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.24
52.58
179.68
27.1
55.07
165.2
Standard deviation
2.75
4.43
18.74
2.55
4.2
13.25
C V (%)
10.48
8.43
10.43
9.41
7.63
8.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat AgRP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat AgRP in samples. No significant cross-reactivity or interference between Rat AgRP and analogues was observed.
