Rat Aldehyde Dehydrogenase, Mitochondrial (ALDM) ELISA Kit
The Rat Aldehyde Dehydrogenase Mitochondrial (ALDHm) ELISA Kit is a powerful tool for researchers studying mitochondrial ALDHm levels in rat samples. This kit offers high sensitivity and specificity, providing accurate and reproducible results in serum, plasma, and cell culture supernatants.Mitochondrial ALDHm is an essential enzyme involved in the detoxification of aldehydes and oxidation of retinol, playing a crucial role in maintaining cellular health and protecting against oxidative stress.
Dysregulation of ALDHm has been linked to various diseases, including cancer, liver disorders, and neurodegenerative conditions, making it a valuable biomarker for understanding and managing these conditions.With its reliable performance and ease of use, the Rat Aldehyde Dehydrogenase Mitochondrial (ALDHm) ELISA Kit is an indispensable tool for researchers seeking to explore the role of mitochondrial ALDHm in health and disease.
Product Name:
Rat Aldehyde Dehydrogenase, Mitochondrial (ALDM) ELISA Kit
Product Code:
RTDL00056
Size:
96 Assays
Target:
ALDM
Synonyms:
ALDH2, ALDH-E2, ALDHI, Aldehyde Dehydrogenase 2
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
