The Rat Apelin-13 (AP13) ELISA Kit is specifically designed for the precise measurement of apelin-13 levels in rat serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Apelin-13 is a key peptide that plays a vital role in regulating various physiological processes, including cardiovascular function, fluid homeostasis, and energy metabolism.
Its dysregulation has been implicated in numerous diseases, such as heart failure, obesity, and diabetes, making it a valuable biomarker for investigating these conditions and exploring potential therapeutic interventions. Overall, the Rat Apelin-13 (AP13) ELISA Kit is an essential tool for researchers looking to study the role of apelin-13 in health and disease, providing reliable data for in-depth analysis and further advancements in scientific knowledge.
Product Name:
Rat Apelin 13 (AP13) ELISA Kit
Product Code:
RTDL00085
Size:
96 Assays
Target:
AP13
Synonyms:
APLN13
Detection Method:
Competition
Range:
93.75-6000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
