Rat BAK1/BCL2-antagonist/killer 1 isoform 1 ELISA Kit
The Rat BAK1 (BCL2 Antagonist/Killer 1) ISOFORM 1 ELISA Kit is a powerful tool for studying the expression levels of BAK1 in rat samples. This kit is specifically designed for the accurate and reliable measurement of BAK1 isoform 1 in serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, researchers can trust the results produced by this kit for a variety of research applications. BAK1 is a key regulator of apoptosis, playing a crucial role in the intrinsic apoptotic pathway by promoting mitochondrial outer membrane permeabilization.
Dysregulation of BAK1 expression has been linked to various diseases, including cancer, making it an important focus for researchers studying cell death mechanisms and potential therapeutic targets. Overall, the Rat BAK1 ISOFORM 1 ELISA Kit provides researchers with a valuable tool for investigating the role of BAK1 in cellular processes and disease states, ultimately contributing to the advancement of scientific knowledge and potential treatment options.
Product Name:
Rat BAK1/BCL2-antagonist/killer 1 isoform 1 ELISA Kit
SKU:
RTFI00591
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.469 ng/mL
Range:
0.781-50 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
92-100%
80-95%
87-95%
EDTA Plasma (n = 5)
84-101%
81-100%
85-102%
Heparin Plasma (n = 5)
86-101%
88-99%
83-95%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
88-101
98
EDTA Plasma (n = 5)
95-101
99
Heparin Plasma (n = 5)
90-101
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
