The Rat BMP7 ELISA Kit is a highly specialized assay designed for the precise measurement of Bone Morphogenetic Protein 7 (BMP7) levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications. BMP7 is a key regulator of bone and cartilage development, as well as being involved in a range of physiological processes such as kidney development, cell differentiation, and tissue repair. Dysregulation of BMP7 has been linked to disorders such as osteoporosis, renal disease, and cancer, making it an important target for research and potential therapeutic interventions.
By utilizing the Rat BMP7 ELISA Kit, researchers can gain valuable insights into the role of BMP7 in various biological processes and disease states, helping to advance our understanding of these conditions and develop new treatment strategies. Trust in the reliability and accuracy of this ELISA kit to support your research endeavors.
Product Name:
Rat BMP7 ELISA Kit
SKU:
RTFI00603
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
9.375 pg/mL
Range:
15.625-1000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1
2
3
Serum (n = 5)
85-102%
84-101%
81-87%
EDTA Plasma (n = 5)
87-98%
82-100%
81-93%
Heparin Plasma (n = 5)
93-100%
87-98%
84-93%
Recovery:
Sample
1
2
Serum (n = 5)
87-97
91
EDTA Plasma (n = 5)
86-103
94
Heparin Plasma (n = 5)
95-103
98
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
