Rat Carboxypeptidase A3, Mast Cell (CPA3) ELISA Kit
The Rat Carboxypeptidase A3 (Mast Cell CPA3) ELISA Kit is a valuable tool for the accurate measurement of carboxypeptidase A3 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring reliable results for a variety of research applications.Carboxypeptidase A3 (CPA3) is an enzyme that plays a critical role in mast cell function, including the processing of bioactive peptides and regulation of inflammatory responses.
Dysregulation of CPA3 has been implicated in various immune disorders and allergic conditions, making it a key target for research and potential therapeutic interventions.With its high-quality components and easy-to-use format, the Rat Carboxypeptidase A3 ELISA Kit is a valuable resource for scientists studying mast cell biology, immune responses, and inflammatory disorders in rat models. Its accurate and reproducible results make it an essential tool for advancing research in this field.
Product Name:
Rat Carboxypeptidase A3, Mast Cell (CPA3) ELISA Kit
Product Code:
RTDL00241
Size:
96 Assays
Target:
CPA3
Synonyms:
MC-CPA, Mast cell carboxypeptidase A
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
