The Rat COL10 (Collagen Type X) CLIA Kit is specifically designed for the quantitative measurement of collagen type X levels in rat samples. This kit offers high sensitivity and accuracy, providing researchers with reliable and reproducible results for various research applications.Collagen type X is a key component of the extracellular matrix and is essential for proper bone formation and skeletal development.
Abnormal levels of collagen type X have been associated with various musculoskeletal disorders, making it a valuable biomarker for studying and potentially treating these conditions.With its easy-to-use format and excellent performance, the Rat COL10 CLIA Kit is a valuable tool for researchers interested in studying collagen type X and its implications in skeletal health and disease.
Product Name:
Rat COL10 (Collagen Type X) CLIA Kit
SKU:
RTES00143
Target:
Rat COL10 (Collagen Type X)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat COL10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat COL10. You can calculate the concentration of Rat COL10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
89-101
103-120
Average (%)
91
96
109
1:4
Range (%)
101-118
103-119
92-108
Average (%)
108
109
98
1:8
Range (%)
87-100
101-117
97-112
Average (%)
92
107
103
1:16
Range (%)
103-117
92-106
87-98
Average (%)
109
97
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
102
EDTA plasma (n=5)
92-105
97
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.69
96.45
292.05
40.55
88.03
300.65
Standard deviation
3.98
9.14
20.47
4.97
6.28
25.65
C V (%)
9.11
9.48
7.01
12.26
7.13
8.53
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat COL10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat COL10 in samples. No significant cross-reactivity or interference between Rat COL10 and analogues was observed.
