The Rat COL3 (Collagen Type III) ELISA Kit is an advanced research tool designed for the quantitative determination of Collagen Type III levels in a variety of rat biological samples. Collagen Type III is a crucial structural protein that plays a significant role in providing structural support and elasticity to connective tissues, including skin, blood vessels, and internal organs. This ELISA kit enables accurate measurement of Collagen Type III concentrations, facilitating insights into tissue remodeling, wound healing, and fibrotic processes. Precise quantification of Collagen Type III levels is vital for understanding its contribution to tissue integrity, elasticity, and repair mechanisms. By utilizing the Rat COL3 ELISA Kit, researchers can investigate the dynamics of Collagen Type III expression, explore its involvement in tissue remodeling processes, and elucidate its significance in various physiological and pathological conditions. The Rat COL3 ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control measures, this kit delivers robust and reliable performance, making it an ideal choice for research projects focused on unraveling the biological roles of Collagen Type III in health and disease.
Product Name:
Rat COL3 (Collagen Type III) ELISA Kit
SKU:
AEES00360
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
