The Mouse COL3 (Collagen Type III) ELISA Kit is specifically designed for the precise measurement of collagen type III levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees accurate and consistent results, making it a valuable tool for various research purposes.Collagen type III is a key component of the extracellular matrix, contributing to tissue integrity and function. Changes in collagen type III levels have been linked to various diseases, including fibrosis, cardiovascular disorders, and connective tissue disorders. This ELISA kit enables researchers to study the role of collagen type III in disease pathology and potential therapeutic interventions.Overall, the Mouse COL3 (Collagen Type III) ELISA Kit offers researchers a reliable and efficient method for investigating the role of collagen type III in health and disease in mouse models.
Product Name:
Mouse COL3 (Collagen Type III) ELISA Kit
SKU:
MOES00880
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
