The Rat Collagen Type IV (COL4) ELISA Kit is a specialized assay designed for the quantitative measurement of Collagen Type IV levels in rat biological samples. Collagen Type IV is a critical structural protein that forms a major component of the basement membrane, contributing to tissue integrity, filtration barrier function, and cell adhesion. This ELISA kit provides researchers with a powerful tool to investigate the regulation of Collagen Type IV in various physiological and pathological conditions. Accurate quantification of Collagen Type IV levels is essential for studying basement membrane biology, extracellular matrix remodeling, and tissue-specific functions. The Rat COL4 ELISA Kit enables researchers to explore the role of Collagen Type IV in tissue development, repair processes, and disease pathogenesis, offering valuable insights into the mechanisms underlying basement membrane-related disorders and tissue homeostasis. By leveraging the exceptional sensitivity and specificity of the Rat COL4 ELISA Kit, researchers can obtain reliable and reproducible results, ensuring high-quality data for comprehensive investigations into Collagen Type IV dynamics and its impact on cellular interactions and tissue function. Trust in the robust performance of this kit to advance research in collagen biology, extracellular matrix regulation, and basement membrane integrity.
Product Name:
Rat COL4 (Collagen Type 4) ELISA Kit
SKU:
AEES00525
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
