The Rat CTSB (Cathepsin B) ELISA Kit is specifically designed for the precise measurement of cathepsin B levels in rat samples including serum, plasma, and cell culture supernatants. This kit offers a high level of sensitivity and specificity, ensuring accurate and consistent results for various research applications.Cathepsin B is an important lysosomal cysteine protease involved in various biological processes including protein degradation, antigen processing, and cell death regulation.
Dysregulation of cathepsin B has been linked to a wide range of diseases such as cancer, cardiovascular diseases, and inflammatory disorders, highlighting its significance as a potential biomarker and therapeutic target.With its reliability and ease of use, the Rat CTSB ELISA Kit provides researchers with a valuable tool for investigating the role of cathepsin B in disease pathogenesis and exploring its potential as a therapeutic target.
Product Name:
Rat CTSB (Cathepsin B) ELISA Kit
SKU:
RTES01042
Target:
Rat CTSB (Cathepsin B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CTSB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTSB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTSB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CTSB. You can calculate the concentration of Rat CTSB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
98-113
95-108
Average (%)
102
105
102
1:4
Range (%)
91-103
82-96
89-101
Average (%)
97
88
95
1:8
Range (%)
92-104
85-98
86-99
Average (%)
99
90
92
1:16
Range (%)
93-103
80-91
88-103
Average (%)
98
85
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
94
EDTA plasma (n=5)
87-99
94
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.54
4.48
0.51
1.6
4.86
Standard deviation
0.03
0.07
0.17
0.03
0.07
0.19
C V (%)
6
4.55
3.79
5.88
4.38
3.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CTSB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CTSB in samples. No significant cross-reactivity or interference between Rat CTSB and analogues was observed.
