The Rat Doublecortin (DCX) ELISA Kit is a reliable and efficient tool for the quantitative measurement of DCX levels in rat samples including serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for a wide range of research applications.Doublecortin is a microtubule-associated protein that plays a crucial role in neuronal migration and differentiation. Dysregulation of DCX expression has been implicated in various neurological disorders, making it a valuable biomarker for studying neurodevelopmental and neurodegenerative conditions.
By using the Rat Doublecortin (DCX) ELISA Kit, researchers can gain valuable insights into the role of DCX in the development and function of the nervous system, as well as its potential implications in neurodegenerative diseases. This kit is an essential tool for advancing our understanding of neuronal development and exploring new therapeutic strategies for neurological disorders.
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
El-Mezayen, N, E., et al.
Vitamin a modulates neurogenesis-associated pathways and cholinergic signaling in Alzheimer’s disease: potential role of reactive astrocytes via NGN2/SOX-11 and SIRT-1
