Rat Fatty Acid Binding Protein 2, Intestinal (FABP2) ELISA Kit
The Rat Fatty Acid Binding Protein 2 (Intestinal FABP2) ELISA Kit is a powerful tool for measuring levels of FABP2 in rat serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it perfect for a variety of research applications.FABP2, also known as Intestinal Fatty Acid Binding Protein, is a key protein involved in the transport and metabolism of fatty acids in the intestine. It plays a crucial role in lipid absorption and metabolism, making it a valuable marker for studying metabolic disorders, gut health, and the impact of diet on overall health.
Whether you are investigating the role of FABP2 in obesity, diabetes, or inflammatory bowel diseases, the Rat FABP2 ELISA Kit provides a reliable and efficient method for quantifying FABP2 levels in rat samples. Trust in this kit to accelerate your research and uncover new insights into the intricate mechanisms of fatty acid transport and metabolism in the intestine.
Product Name:
Rat Fatty Acid Binding Protein 2, Intestinal (FABP2) ELISA Kit
Product Code:
RTDL00352
Size:
96 Assays
Target:
FABP2
Synonyms:
FABPI, IFABP, I-FABP, Intestinal-type fatty acid-binding protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
