Mouse Fatty Acid Binding Protein 2, Intestinal (FABP2) ELISA Kit
The Mouse Fatty Acid Binding Protein 2 (Intestinal FABP2) ELISA Kit is a highly specific and sensitive assay designed for the quantitative measurement of FABP2 levels in mouse serum, plasma, and tissue homogenates. This kit offers reliable and reproducible results, making it an essential tool for researchers studying lipid metabolism and intestinal function in mouse models.FABP2, also known as intestinal fatty acid-binding protein, is a key protein involved in the transport and metabolism of fatty acids in the intestine.
It plays a crucial role in lipid absorption, storage, and utilization, making it a valuable biomarker for investigating metabolic disorders, inflammatory bowel diseases, and obesity-related conditions in mice.With its high performance and accuracy, the Mouse FABP2 ELISA Kit is suitable for a wide range of research applications, providing valuable insights into the role of FABP2 in mouse physiology and disease pathology.
Product Name:
Mouse Fatty Acid Binding Protein 2, Intestinal (FABP2) ELISA Kit
Product Code:
MODL00444
Size:
96 Assays
Target:
FABP2
Synonyms:
FABPI, IFABP, I-FABP, Intestinal-type fatty acid-binding protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
