The Rat FGF21 ELISA Kit is a precise and reliable tool for the measurement of fibroblast growth factor 21 (FGF21) levels in rat samples including serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications. FGF21 is a key metabolic regulator that plays a crucial role in regulating energy balance, glucose homeostasis, and lipid metabolism. Dysregulation of FGF21 has been linked to various metabolic disorders such as obesity, diabetes, and fatty liver disease, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.
With this Rat FGF21 ELISA Kit, researchers can confidently study the role of FGF21 in metabolic diseases and explore its potential as a target for novel treatments. Its user-friendly protocol and superior performance make it an essential tool for advancing research in the field of metabolic disorders.
Product Name:
Rat FGF21 ELISA Kit
SKU:
RTFI00775
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-95%
82-101%
80-100%
EDTA Plasma (n = 5)
97-103%
82-100%
88-100%
Heparin Plasma (n = 5)
86-99%
91-100%
90-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-101
95
EDTA Plasma (n = 5)
89-105
97
Heparin Plasma (n = 5)
86-101
90
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
