The Rat FN (Fibronectin) CLIA Kit is a powerful tool for measuring levels of fibronectin in rat biological samples such as serum, plasma, and tissue culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reliable results for various research applications.Fibronectin is a key extracellular matrix protein that plays a crucial role in cell adhesion, migration, and tissue remodeling processes. Dysregulation of fibronectin expression has been linked to various diseases, including cancer, fibrosis, and inflammatory conditions. Therefore, studying fibronectin levels can provide valuable insights into disease progression and therapeutic strategies.This Rat FN CLIA Kit offers a convenient and efficient way to quantify fibronectin levels in rat samples, allowing researchers to explore the role of fibronectin in various physiological and pathological processes. Get reliable data to advance your research with this high-quality assay kit from AssayGenie.
Product Name:
Rat FN (Fibronectin) CLIA Kit
SKU:
RTES00355
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
