The Rat FN (Fibronectin) ELISA Kit is a specialized assay designed for the precise quantitative measurement of Fibronectin levels in various rat biological samples. Fibronectin is a key glycoprotein that plays a vital role in cell adhesion, migration, growth, and differentiation. Its involvement in the extracellular matrix and cellular processes makes it a critical player in tissue development, wound healing, and various physiological functions. This ELISA kit, developed by Assay Genie, is equipped with high sensitivity and specificity to ensure accurate and reproducible results. Manufactured under strict quality standards, this kit offers robust performance and user-friendly protocols, making it an excellent choice for researchers studying the role of Fibronectin in rat models and its implications in cellular functions and tissue homeostasis.
Product Name:
Rat FN (Fibronectin) ELISA Kit
SKU:
AEES00463
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
