The Human FN (Fibronectin) ELISA Kit is specifically created for the precise measurement of fibronectin levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures dependable and consistent results, making it suitable for a diverse array of research purposes.Fibronectin is an essential glycoprotein that plays a key role in cell adhesion, growth, migration, and differentiation. It is involved in various physiological processes such as wound healing, embryonic development, and tissue repair. Abnormal fibronectin levels are associated with conditions like cancer, fibrosis, and inflammatory diseases, highlighting its importance as a biomarker for investigating these disorders and potential therapeutic interventions.Get accurate and reliable results with the Human FN (Fibronectin) ELISA Kit, and unlock new insights into the role of fibronectin in health and disease.
Product Name:
Human FN (Fibronectin) ELISA Kit
SKU:
HUES01446
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
