Rat Glycoprotein 2, Zymogen Granule Membrane (GP2) ELISA Kit
The Rat Glycoprotein 2 (GP2) Zymogen Granule Membrane ELISA Kit is a powerful tool for researchers looking to accurately measure levels of GP2 in rat samples. This kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.GP2 is a key protein found on zymogen granule membranes, playing a crucial role in digestive enzyme secretion and gut immunity.
Understanding GP2 levels in rat models can provide valuable insights into digestive disorders, immune responses, and potential therapeutic targets.With the Rat GP2 ELISA Kit, researchers can confidently study the involvement of GP2 in various physiological and pathological processes, advancing our knowledge of digestive health and immune function in rats.
Product Name:
Rat Glycoprotein 2, Zymogen Granule Membrane (GP2) ELISA Kit
Product Code:
RTDL00448
Size:
96 Assays
Target:
GP2
Synonyms:
ZAP75, Pancreatic Secretory Granule Membrane Major Glycoprotein GP2, Pancreatic zymogen granule membrane protein GP-2
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
