Rat HMGB-1 (High Mobility Group Protein B1) CLIA Kit
The Rat HMGB-1 (High Mobility Group Protein B1) CLIA Kit is a highly reliable and accurate assay kit designed to detect HMGB-1 levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it suitable for a wide range of research applications.HMGB-1 is a key protein involved in inflammatory responses, DNA repair, and cell differentiation. Elevated levels of HMGB-1 have been associated with various diseases such as sepsis, arthritis, and cancer, making it a valuable biomarker for studying these conditions and potentially developing therapeutic interventions.
With the Rat HMGB-1 CLIA Kit, researchers can confidently analyze HMGB-1 levels in rat samples, furthering their understanding of HMGB-1 biology and its role in disease progression. Trust in the accuracy and precision of this kit for your research needs.
Product Name:
Rat HMGB-1 (High Mobility Group Protein B1) CLIA Kit
SKU:
RTES00299
Target:
Rat HMGB-1 (High Mobility Group Protein B1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HMGB-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HMGB-1. You can calculate the concentration of Rat HMGB-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
91-105
92-108
Average (%)
97
96
99
1:4
Range (%)
97-111
92-106
95-107
Average (%)
103
97
101
1:8
Range (%)
89-104
93-106
102-117
Average (%)
96
98
108
1:16
Range (%)
103-115
102-115
97-109
Average (%)
109
108
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-117
107
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
94-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
44.19
90.41
349.84
41.54
91.66
355.69
Standard deviation
5.62
7.74
38.45
4.29
10.44
31.41
C V (%)
12.72
8.56
10.99
10.33
11.39
8.83
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HMGB-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HMGB-1 in samples. No significant cross-reactivity or interference between Rat HMGB-1 and analogues was observed.
