The Rat HO1 (Heme Oxygenase 1) ELISA Kit is a cutting-edge assay designed for the precise quantification of Heme Oxygenase 1 levels in various rat biological samples. Heme Oxygenase 1 is a crucial enzyme involved in heme catabolism, playing a pivotal role in maintaining cellular homeostasis and modulating oxidative stress responses. By quantifying HO1 levels, researchers can gain valuable insights into the cellular stress response, antioxidant defense mechanisms, and the regulation of heme metabolism. HO1 is a key player in the antioxidant defense system, serving as a cytoprotective enzyme that degrades heme into carbon monoxide, biliverdin, and iron, with potent anti-inflammatory, anti-apoptotic, and cytoprotective functions. Its upregulation in response to various cellular stressors underscores its importance in mitigating oxidative damage and promoting cellular resilience in the face of environmental challenges. Accurate measurement of HO1 levels using the Rat HO1 ELISA Kit provides researchers with a comprehensive tool to study oxidative stress, redox signaling pathways, and the adaptive cellular response to various physiological and pathological conditions. With exceptional sensitivity and specificity, this kit offers reliable and reproducible results, making it an indispensable asset for unraveling the intricate roles of HO1 in cellular protection, redox homeostasis, and disease pathogenesis.
Product Name:
Rat HO1 (Heme Oxygenase 1) ELISA Kit
SKU:
AEES00428
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
95-112
95-111
93-108
Average (%)
103
103
99
1:4
Range (%)
88-104
85-98
88-101
Average (%)
95
90
94
1:8
Range (%)
86-102
83-96
85-96
Average (%)
93
89
91
1:16
Range (%)
89-101
85-97
90-103
Average (%)
95
91
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
90-103
98
Cell Culture Media (n=8)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.38
4.65
0.53
1.35
4.49
Standard deviation
0.02
0.06
0.28
0.05
0.1
0.27
C V (%)
4.02
4.44
6.12
8.99
7.41
6.1
Uniport ID:
P06762
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Rat HO1 in samples. No significant cross-reactivity or interference between Rat HO1 and analogues was observed
Storage:
2-8℃
Research Area:
Cancer,Cardiovascular,Epigenetics And Nuclear Signaling,Metabolism,Neuroscience,Signal Transduction
Abdallah, M.H et al.
Omarigliptin/shikonin combination alleviates cyclosporine-induced nephrotoxicity: The role of sirtuin 1, glucagon-like peptide-1, HMGB1/RAGE/ TLR4 signaling, and p38/ERK/JNK MAPKs
