The Human HO-1 (Heme Oxygenase-1) ELISA Kit is a specialized assay designed for the precise measurement of HO-1 levels in human samples such as serum, plasma, and cell culture supernatants. This cutting-edge kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.HO-1 is a critical enzyme involved in the breakdown of heme and the maintenance of cellular homeostasis. Its dysregulation has been implicated in numerous health conditions, including oxidative stress-related diseases, inflammatory disorders, and various cancers. Therefore, monitoring HO-1 levels can provide valuable insights into the pathogenesis of these diseases and facilitate the development of novel therapeutic strategies.Elevated HO-1 expression has also been associated with protective effects against tissue damage and inflammation, highlighting its potential as a biomarker for assessing disease severity and prognosis. With its advanced features and reliable performance, the Human HO-1 ELISA Kit is an indispensable tool for researchers seeking to delve deeper into the physiological and pathological roles of HO-1 in human health and disease.
Product Name:
Human HO1 (Heme Oxygenase 1) ELISA Kit
SKU:
HUES03073
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
