The Rat HSP 40 (Heat Shock Protein 40) CLIA Kit is specifically designed for the quantification of HSP 40 levels in rat samples. This kit offers exceptional sensitivity and accuracy, providing reliable and consistent results for various research applications.HSP 40 is a key molecular chaperone involved in protein folding and cellular stress response. It plays a crucial role in maintaining cellular homeostasis and protecting cells from damage, making it an important target for studying diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases.
With the Rat HSP 40 CLIA Kit, researchers can easily and efficiently measure HSP 40 levels in serum, plasma, or cell culture supernatants, allowing for in-depth investigations into its function and potential therapeutic implications. Trust in this kit for robust and precise quantification of HSP 40 levels in rat samples.
Product Name:
Rat HSP-40 (Heat Shock Protein 40) CLIA Kit
SKU:
RTES00277
Target:
Rat HSP-40 (Heat Shock Protein 40)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HSP-40. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSP-40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSP-40, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HSP-40. You can calculate the concentration of Rat HSP-40 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
103-116
89-101
Average (%)
104
110
94
1:4
Range (%)
97-113
85-98
101-114
Average (%)
103
91
106
1:8
Range (%)
102-116
88-104
85-100
Average (%)
109
95
92
1:16
Range (%)
99-116
97-115
97-111
Average (%)
106
105
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-117
107
EDTA plasma (n=5)
94-110
101
Cell culture media (n=5)
94-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
182.61
350.48
1622.51
195.92
360.47
1585.44
Standard deviation
17.0
39.15
163.71
22.41
32.33
129.53
C V (%)
9.31
11.17
10.09
11.44
8.97
8.17
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HSP-40 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HSP-40 in samples. No significant cross-reactivity or interference between Rat HSP-40 and analogues was observed.
