The Rat IgE (Immunoglobulin E) ELISA Kit is specifically developed to quantitatively measure the levels of Immunoglobulin E (IgE) in a variety of biological samples. IgE is a type of antibody that plays a crucial role in allergic responses and helps protect against parasite infections. When an individual with a hypersensitivity or allergy is exposed to an allergen, the body produces IgE antibodies that trigger the release of histamine and other mediators, leading to allergic symptoms. Accurate detection and quantification of IgE levels are essential for diagnosing allergies and monitoring immune responses. With Assay Genie's Rat IgE ELISA Kit, researchers can achieve reliable, reproducible, and precise results. This kit is designed with exceptional sensitivity and specificity, ensuring high-quality performance and ease of use, making it an ideal choice for allergy research and immunological studies.
Product Name:
Rat IgE (Immunoglobulin E) ELISA Kit
SKU:
AEES00439
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
