Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit
The Rat Insulin-Like Growth Factor Binding Protein 3 (IGFBP-3) ELISA Kit is a specialized assay designed to quantitatively detect and measure the levels of IGFBP-3 in various biological samples from rats. IGFBP-3 plays a crucial role in modulating the activity and bioavailability of insulin-like growth factors, particularly IGF-1, which are essential for cell growth and proliferation. This ELISA kit enables researchers to accurately determine IGFBP-3 concentrations, providing valuable insights into the regulation of IGF signaling pathways, cell growth control, and overall metabolic functions. By offering exceptional sensitivity and specificity, this kit allows for precise and reliable measurement of IGFBP-3 levels, crucial for understanding its involvement in growth regulation, development processes, and potential implications in various physiological and pathological conditions. The Rat IGFBP-3 ELISA Kit by Assay Genie delivers robust and reproducible results, ensuring data accuracy and consistency in research studies focused on IGF-related mechanisms and growth factor signaling pathways. Manufactured under stringent quality control standards, this kit offers high performance and user-friendly protocols, making it an ideal choice for researchers investigating the complex interactions of IGFBP-3 in growth and development processes.
Product Name:
Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit
SKU:
AEES00531
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
