The Rat IL12-p40 ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of interleukin-12 (IL-12) p40 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications in the fields of immunology and inflammation. IL-12 p40 is a subunit of the pro-inflammatory cytokine IL-12, which plays a crucial role in the regulation of immune responses. It is involved in the differentiation of T cells and the production of interferon gamma, making it a key player in the immune system's ability to fight infection and regulate inflammation.
Elevated levels of IL-12 p40 have been implicated in various inflammatory conditions, autoimmune diseases, and infectious diseases, making it a valuable biomarker for understanding the pathogenesis of these conditions. With its high sensitivity and specificity, the Rat IL12-p40 ELISA Kit is an invaluable tool for researchers studying the role of IL-12 p40 in health and disease. Its accurate quantification of IL-12 p40 levels allows for precise measurement of cytokine levels in biological samples, enabling further insights into the mechanisms underlying immune responses and inflammation.
Product Name:
Rat IL-12 (p40) ELISA Kit
SKU:
RTFI00899
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
7.031 pg/mL
Range:
11.719-750 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-100%
83-99%
84-100%
EDTA Plasma (n = 5)
85-103%
87-100%
81-94%
Heparin Plasma (n = 5)
86-101%
92-99%
82-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-105
91
EDTA Plasma (n = 5)
85-99
92
Heparin Plasma (n = 5)
85-102
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
