The Rat IL1R1 ELISA Kit (RTES01065) from Assay Genie is a powerful tool for accurately measuring levels of IL1R1 in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it suitable for a diverse range of research applications.IL1R1, also known as Interleukin-1 receptor type 1, plays a critical role in the immune response and inflammation. By detecting IL1R1 levels, researchers can gain valuable insights into the mechanisms underlying various diseases, including autoimmune disorders, inflammatory conditions, and infectious diseases. This kit is an essential resource for studying the role of IL1R1 in health and disease, advancing our understanding of immune regulation and potential therapeutic targets. Get your Rat IL1R1 ELISA Kit (RTES01065) from Assay Genie today and unlock new possibilities in your research.
Product Name:
Rat IL-1R1 ELISA Kit
SKU:
RTES01065
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
