The Rat Inhibin B ELISA Kit is a reliable and accurate tool designed for the detection of inhibin B levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Inhibin B is a key protein involved in regulating reproductive functions, especially in the control of follicle-stimulating hormone (FSH) levels and the development of healthy sperm in males. It plays a critical role in various reproductive disorders and infertility, making it a valuable biomarker for studying these conditions and potential treatment strategies.Overall, the Rat Inhibin B ELISA Kit is an essential tool for researchers studying reproductive health and fertility in rats, providing accurate and reliable quantification of inhibin B levels for impactful research outcomes.
Product Name:
Rat INHB (Inhibin B) ELISA Kit
SKU:
RTES00859
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
