Rat LDL-C (low density lipoprotein cholesterol) ELISA Kit
The Rat LDL-C (Low-Density Lipoprotein Cholesterol) ELISA Kit is a reliable and accurate assay designed for the quantitative measurement of LDL-C levels in rat serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring precise and consistent results for a variety of research applications. Low-density lipoprotein cholesterol (LDL-C) is a key component of the lipid profile and plays a crucial role in the development of cardiovascular diseases. Elevated levels of LDL-C are associated with an increased risk of atherosclerosis, heart disease, and stroke.
Monitoring LDL-C levels is essential for understanding cholesterol metabolism and assessing cardiovascular risk. This ELISA kit provides researchers with a valuable tool for studying lipid metabolism, cardiovascular health, and the effects of dietary interventions or pharmaceutical treatments on LDL-C levels in rat models. With its high-quality components and easy-to-follow protocol, the Rat LDL-C ELISA Kit is an indispensable resource for investigating the role of LDL-C in health and disease.
Product Name:
Rat LDL-C (low density lipoprotein cholesterol) ELISA Kit
SKU:
AEFI00959
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.469 ng/mL
Range:
0.781-50 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
88-105%
87-97%
82-98%
EDTA Plasma (n = 5)
85-100%
84-100%
81-100%
Heparin Plasma (n = 5)
92-105%
86-95%
85-91%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
88-101
96
EDTA Plasma (n = 5)
85-101
92
Heparin Plasma (n = 5)
87-103
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
