Rat Lipopolysaccharide Binding Protein (LBP) ELISA Kit
The Rat Lipopolysaccharide Binding Protein (LBP) ELISA Kit is specifically designed for the quantitative detection of LBP levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.LBP is a key protein that plays a critical role in the innate immune response by binding to bacterial lipopolysaccharides, triggering the inflammatory cascade. Dysregulation of LBP levels has been associated with various inflammatory conditions, making it a valuable biomarker for studying infectious diseases, sepsis, and other inflammatory disorders.
By utilizing the Rat LBP ELISA Kit, researchers can gain valuable insights into the role of LBP in the immune response and potential therapeutic strategies for targeting LBP-mediated inflammatory pathways. This kit provides a reliable tool for studying LBP in rat models, advancing our understanding of inflammatory mechanisms and developing novel treatments.
Product Name:
Rat Lipopolysaccharide Binding Protein (LBP) ELISA Kit
Product Code:
RTDL00620
Size:
96 Assays
Target:
LBP
Synonyms:
LPS-Binding Protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
Siriwan Chumroenvidhayakul et al.
MODULATION OF COLONIC BACTERIAL METABOLITES AND MUCOSAL BARRIER BY DRAGON FRUIT PEEL WASTE (HYLOCEREUS UNDATUS) IN A HIGH-FAT, HIGH-FRUCTOSE DIET-INDUCED RAT MODEL
