Rat LBP (Lipopolysaccharide Binding Protein) ELISA Kit
The Rat LBP (Lipopolysaccharide Binding Protein) ELISA Kit is a powerful tool designed for the precise quantification of Lipopolysaccharide Binding Protein in rat samples. LBP is an essential component of the host defense mechanism, acting as a pattern recognition receptor involved in the immune response to bacterial infections. Its ability to bind and neutralize lipopolysaccharides (endotoxins) plays a pivotal role in innate immunity and inflammatory processes. Accurate measurement of LBP levels is crucial for understanding its function in host defense, infection, and inflammation. Our ELISA kit ensures exceptional sensitivity and specificity, enabling dependable and reproducible results for your research requirements. Manufactured with stringent quality control measures, this kit delivers robust performance and user-friendly operation, making it an ideal choice for investigating the role of LBP in immune responses and inflammatory pathways. Trust Assay Genie's ELISA kits for high-quality and accurate measurement of key biomarkers in your research studies.
Product Name:
Rat LBP (Lipopolysaccharide Binding Protein) ELISA Kit
SKU:
AEES00468
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.13 ng/mL
Detection range:
1.88-120 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.